Terrestrial Vertebrates (Mouse Facility)
The terrestrial vertebrate facility(Mouse Facility) of the CABD has the capacity to house approximately 6,000 mice. In an area of over 300 m2. The facility comprises five separated units subdivided each into procedure and housing rooms and two rooms for quarantine. The facility also contains X-Ray equipment.
The CABD backs the Agreement on Openness on Animal Research.
The CABD backs the Agreement on Openness on Animal Research.
1. EQUIPMENT
.jpg)
Currently, the mouse house at CABD has 15 ventilated racks of two diferents models, 8 for 64 microisolators (Techniplast) and 9 for 81 microisolators (Allentown) connected ventilation towers. These systems are available in all rooms of the facility. The facility also has a metabolic rack (TSE System, Pheomaster calo).The transgenic service has a LEICA DM-IRB microscope for pronuclear injection, three stereo-microscopes for embryo isolation and micro-surgery, two CO2 incubators, a micro-puller to produce microinjection needles and a micro-forge, for modelling borosilicate capillaries.
The facility also has an Animal Irradiation service (Encapsulated sources).
Facility telephone number: +34 618865482 (ext. interna UPO 2482)
Animal House Director: Dr Javier Lopez-Rios
Senior technician: Ana J. Franco Gómez
Support technical personnel: Candida Mateos Orozco
Facility telephone number: +34 618865482 (ext. interna UPO 2482)
Animal House Director: Dr Javier Lopez-Rios
Senior technician: Ana J. Franco Gómez
Support technical personnel: Candida Mateos Orozco
Mice microisolators
Metabolic rack
Animal Irradiation (X-Ray)
Animal Irradiation (X-Ray)
2. SERVICES
Collaborations, advice, consulting and contract services are offered on the following techniques:
A. TRANSGENESIS
The transgenic services unit offers:
- DNA extraction and purification of constructs for microinjection
- Generation of expression vectors for transgenesis
- Generation of F0 embryos and adult animals as well as establishment of new stable transgenic lines
B. GENOTYPING
- Extraction and purification of biopsy DNA for genotyping purposes
- Optimisation of existing genotyping protocols
- Generation of new genotyping protocols for individual lines
