Venue:
Aquatic Vertebrate Platform
Centro Andaluz de Biologia del Desarrollo-CSIC-UPO
Campus de la Universidad Pablo de Olavide
Edificio "J. A. Campos-Ortega"
Crta. Utrera km.1
41013 Sevilla, Spain
How to get there: map |
|
Title: 5th CABD workshop: Applied Technologies for Aquatic Vertebrates
Dates: June 5-8, 2017
Download flyer
Organizer: Ana Fernández-Miñán
Scientific Manager of the Aquatic Vertebrates Platform
mail: amfermin@upo.es
tel: +34 954 977445
web:
Aquatic Vertebrates Platform
Registration Opens Date: Wednesday, April 5, 2017
Registration Deadline: Monday, May 8, 2017
Acceptance Notification Date: Tuesday, May 9, 2017
Participation: Limited number of participants.
Registration Fee: 130 Euros
To register please send the following information to amfermin@upo.es
First Name
Family Name
Lab/Institute
PhD/Post-doc/Other
Nationality
e-mail address
Have you got previous experience with zebrafish? Yes/No
PRELIMINARY PROGRAMME
4th CABD Workshop on Applied Technologies in Aquatic Vertebrates
Time |
Topic |
DAY 1 |
Monday June 5, 2017 |
09:30-10:30 |
Talk: Teleosts as Genetic Models |
10:30-11:00 |
Tutorial: Micro-injection in Zebrafish |
11:00-13:00 |
Practical Session I. Fish facilities and set-up |
|
|
DAY 2 |
Tuesday June 6, 2017 |
09:30-13:00 |
Practical Session II: Micro-injection techniques in zebrafish |
13:00-15:00 |
Lunch |
15:00-17:00 |
Practical Session III. Zebrafish husbandry |
|
|
DAY 3
|
Wednesday June 7, 2017 |
09:30-12:00 |
Practical Session II: Micro-injection techniques in zebrafish |
12:00-13:00 |
Practical Session IV. Transgenic embryos visualisation |
|
|
DAY 4
|
Thursday June 8, 2017 |
09:30-10:30 |
Practical Session IV. Transgenic embryos visualisation |
10:30-14:00 |
Practical Session V. Confocal time-lapse microscopy |
Practical session I. Fish facilities and set-up.
- Visit to the fish facilities.
- Set-up for injection.
Scientific Manager AVP: Ana Fernández-Miñán
Practical session II. Micro-injection techniques in zebrafish.
- Set up zebrafish adults for crosses
- One-cell stage embryos collection
- Tol2-mediated transgenesis
- mRNA and Morpholino injections
- CRISPR/Cas9 injections
Scientific Manager AVP: Ana Fernández-Miñán
Practical session III. Zebrafish husbandry.
- Husbandry
- Feeding. Auxiliary cultures (arthemia, rotifers).
- Water condition analysis (pH, nitrites, nitrates, conductivity).
- Zoonosis. Bleaching of embryos.
Fish Facility Senior Technician: Rocío Morales-Falcón
Practical session IV. Transgenic embryos visualization with fluorescence scopes.
- Evaluation of the injected embryos on practical session III.
- Image acquisition of transgenic zebrafish embryos.
Scientific Manager AVP: Ana Fernández-Miñán
Practical session V. Confocal time-lapse microscopy.
- Selection of transgenic embryos for microscopy
- Embryo mounting for confocal microscopy
- Time-lapse microscopy
Scientific Manager AVP: Ana Fernández-Miñán
If you have any queries, please do not hesitate to contact us.