Centro Andaluz de Biología del Desarrollo
5thCABD Workshop: Applied Technologies for Aquatic Vertebrates
| Venue: Aquatic Vertebrate Platform Centro Andaluz de Biologia del Desarrollo-CSIC-UPO Campus de la Universidad Pablo de Olavide Edificio "J. A. Campos-Ortega" Crta. Utrera km.1 41013 Sevilla, Spain How to get there: map |
Title: 5th CABD workshop: Applied Technologies for Aquatic Vertebrates
Dates: June 5-8, 2017
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Organizer: Ana Fernández-Miñán
Scientific Manager of the Aquatic Vertebrates Platform
mail: amfermin@upo.es
tel: +34 954 977445
web: Aquatic Vertebrates Platform
Registration Opens Date: Wednesday, April 5, 2017
Registration Deadline: Monday, May 8, 2017
Acceptance Notification Date: Tuesday, May 9, 2017
Participation: Limited number of participants.
Registration Fee: 130 Euros
To register please send the following information to amfermin@upo.es
First Name
Family Name
Lab/Institute
PhD/Post-doc/Other
Nationality
e-mail address
Have you got previous experience with zebrafish? Yes/No
PRELIMINARY PROGRAMME
| Time | Topic |
| DAY 1 | Monday June 5, 2017 |
| 09:30-10:30 | Talk: Teleosts as Genetic Models |
| 10:30-11:00 | Tutorial: Micro-injection in Zebrafish |
| 11:00-13:00 | Practical Session I. Fish facilities and set-up |
| DAY 2 | Tuesday June 6, 2017 |
| 09:30-13:00 | Practical Session II: Micro-injection techniques in zebrafish |
| 13:00-15:00 | Lunch |
| 15:00-17:00 | Practical Session III. Zebrafish husbandry |
| DAY 3 |
Wednesday June 7, 2017 |
| 09:30-12:00 | Practical Session II: Micro-injection techniques in zebrafish |
| 12:00-13:00 | Practical Session IV. Transgenic embryos visualisation |
| DAY 4 |
Thursday June 8, 2017 |
| 09:30-10:30 | Practical Session IV. Transgenic embryos visualisation |
| 10:30-14:00 | Practical Session V. Confocal time-lapse microscopy |
Practical session I. Fish facilities and set-up.
- Visit to the fish facilities.
- Set-up for injection.
Scientific Manager AVP: Ana Fernández-Miñán
Practical session II. Micro-injection techniques in zebrafish.
- Set up zebrafish adults for crosses
- One-cell stage embryos collection
- Tol2-mediated transgenesis
- mRNA and Morpholino injections
- CRISPR/Cas9 injections
Scientific Manager AVP: Ana Fernández-Miñán
Practical session III. Zebrafish husbandry.
- Husbandry
- Feeding. Auxiliary cultures (arthemia, rotifers).
- Water condition analysis (pH, nitrites, nitrates, conductivity).
- Zoonosis. Bleaching of embryos.
Fish Facility Senior Technician: Rocío Morales-Falcón
Practical session IV. Transgenic embryos visualization with fluorescence scopes.
- Evaluation of the injected embryos on practical session III.
- Image acquisition of transgenic zebrafish embryos.
Scientific Manager AVP: Ana Fernández-Miñán
Practical session V. Confocal time-lapse microscopy.
- Selection of transgenic embryos for microscopy
- Embryo mounting for confocal microscopy
- Time-lapse microscopy
Scientific Manager AVP: Ana Fernández-Miñán
If you have any queries, please do not hesitate to contact us.