1. EQUIPMENT 2. PROTEIN EXPRESSION AND PURIFICATION 3. SERVICES OFFERED 4. RATES |
5. FACILITY CONTACT 6. UPO LIBRARY RESOURCES 7. EDUCATIONAL WEB PAGES |
Name | Tag | Location |
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pET30a+ | 6His | 02 |
pET9a | 03 | |
pET9b | 04 | |
pET9c | 05 | |
pET9d | 06 | |
pGEX 3X | GST | 10 |
pGEX 2T | GST | 11 |
pGEX 5X1 | GST | 12 |
pTXB1 | Intein | 13 |
pTYB21 | Intein | 14 |
pET14b | 6His | 15 |
pQE30 | 6His | 16 |
Name | Genotype | Location |
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BL21 DE3 pLysS | F– ompT hsdS(rB– mB–) gal dcm λ(DE3) pLysS (CamR )(λ(DE3): lacI, lacUV5-T7 gene 1, ind1, sam7, nin5 ) | 01 |
NCM631-pIZ227 | hsdS gal _DE3:lacI, lacUV5::gen1 (T7 RNA polymerase) _lac linked to Tn10. | 07 |
Rossetta pRare | F– ompT hsdSB(rB– mB–) gal dcm (DE3) pRARE2 (CamR) | 08 |
Rossetta pRare pLysS | F– ompT hsdSB(rB– mB–) gal dcm (DE3) pLysSRARE2 (CamR) | 09 |
Rates (€, IVA not included) |
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CABD-UPO |
OPIs |
Private |
Sample preparation |
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Chemical lysis |
1 |
1.5 |
2 |
Chemical lysis plus grinding |
7.5 |
9.5 |
11.5 |
Sample cleaning (1) |
5.5 |
7 |
9 |
Standard quantitation (2) |
1.5 |
2 |
2.5 |
Protein quantitation (3) |
1 |
1.5 |
2 |
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1-D electrophoresis |
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Minigel (8x7 cm) |
1 |
1.5 |
2 |
Tank Buffer 1L |
2.5 |
3.5 |
4 |
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2-D electrophoresis (4) |
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Minigel (8x7 cm) |
10 |
12.5 |
15.5 |
Midigel (10-18 x 22 cm) |
17.5 |
22 |
27.5 |
Maxigel (26x22 cm) |
25 |
31.5 |
39.5 |
DIGE maxigel(6) |
270 |
337.5 |
422 |
IEF with Manifold (1-12 strips) |
8 |
10 |
12.5 |
Tank Buffer 1L |
2.5 |
3.5 |
4 |
Gel staining (4) |
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Coomassie minigel |
3.5 |
4.5 |
5.5 |
Coomassie maxigel |
15 |
19 |
22.5 |
Fluorescent staining minigel |
16 |
20 |
25 |
Fluorescent staining maxigel |
158 |
198 |
247 |
Silver staining minigel |
4 |
5 |
6.5 |
Silver staining midigel |
15 |
19 |
23.5 |
Silver staining maxigel |
18.5 |
23.5 |
29 |
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FPLC (5) |
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Day of use |
5 |
6.5 |
8 |
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BIACORE (5) |
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Day of use |
10 |
12.5 |
16 |
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(1) Cleaning is made with the 2-D Clean Up kit. Solubilization buffers are included in the price |
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(2) Standard quantitation is needed once for each quantitation |
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(3) Price is for one sample with two replicas. |
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(4) Prices include Loading buffers |
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(5) Chemicals, columns, chips, etc…are not included in the price |
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(6) Image acquisition and data analysis included |
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