The cell culture laboratory is indicated for the work of primary cultures of human cells, mouse cells and for the cultivation of established lines. The use of viruses and vectors will be restricted only to personnel authorized for such use. Mainly, they will have access permission, the vectors that can be used in a P2 and that have also been approved by their service managers, giving their previous approval to work in a room of level P2.
The cell culture room P2 is equipped with the basic material necessary to obtain and maintain cell cultures in a laboratory.
1- Microscope
• ZEISS Celldiscoverer 7
• Automatic sample container recognition
• Barcode reader
• Focus stabilization
• Magnification changer 0.5x / 1x / 2x
• Apochromatic FL beampath with adaptive field stop
• ZEISS Axiocam 506 mono
• Additional camera port
• On-axis access for dispensing
• UV-disinfection
2- Objectives
• Plan-APOCHROMAT 5x / 0.35
• Plan-APOCHROMAT 20x / 0.7 autocorr
• Plan-APOCHROMAT 20x / 0.95 autocorr
• Plan-APOCHROMAT 50x / 1.2 W autocorr
• Autoimmersion
3- Illumination
• Transmitted light unit: IR-LED (725 nm) brightfield, oblique illumination, phase gradient contrast
• Fluorescence: LEDs 385, 420, 470, 520, 567, 590 and 625 nm; High-efficiency multibandpass filter sets; Additional user-accessible emission filter Wheel
4- Accessories
• Temperature and atmospheric control (heating / cooling; CO2, O2)
• Mounting frames for microwell plates, incubations and perfusion
• Insert plates for dishes, multi-chamber slides and standard slides
5- Software
• ZEN 2.3 celldiscoverer includes modules for multi-dimensional image acqusition, Tiles & Positions, Experiment Designer, advanced image processing and analysis tools
The Cell Culture Service of the CABD is equipped by the following instruments:
1. Two biological safety cabinets type II:
Bio II advance 3 of Telstar, one of which is exclusively for work with viruses.
3. CO2 Incubator Nuaire NU58416
4. CO2 installation: The equipment of the incubators is complemented by a CO2 installation
that ensures the constant supply of CO2 to the incubators, making an automatic change of the bottles.
5. Tabletop centrifuge multitasking - Allegra X-12 (BECKMAN COULTER):
maximum speed 10.200 rpm. Maximum capacity 3.000 ml
6. Automatic hand cell counter (Millipore)
7. An tabletop autoclave and a bathroom
And also:
8. An inverted microscope of transmitted light with 10x, 20x and 40x lenses, Leica brand
9. A vacuum pump
10. A refrigerator / freezer