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Cell Culture Facility



1.    DESCRIPTIÓN

2.    EQUIPMENT




CELL CULTURE FACILITY


1. DESCRIPTION

In the Cell Culture Service of the CABD, you can perform, following the current rules, experimental procedures of primary cultures of human cells, mouse cells and established cell lines. Both cell lines and primary cultures can be genetically modified by transfection or infection in the medium of viral vectors.

The Cell Culture Facility gives technical support to the research groups of the CABD.

The service has a cell culture room P2 and a high resolution microscopy equipment for in vivo monitoring of cultures (Celldiscoverer7).


Technical support: Corín Díaz Ramos


Available services

Control of users' access (record of authorized users).                              
Technical advice to the users who require it.                                        
Collaboration in the elaboration / updating of the rules for the use of the cell culture rooms. Supervision of the fulfillment of these rules.
Assignment of equipment and shifts of use / cleaning of these equipment
Preventive maintenance/ cleaning of specific equipment etc.
Maintenance in culture of cell lines.
 


a. Cell Culture Room P2
 features

The cell culture laboratory is indicated for the work of primary cultures of human cells, mouse cells and for the cultivation of established lines. The use of viruses and vectors will be restricted only to personnel authorized for such use. Mainly, they will have access permission, the vectors that can be used in a P2 and that have also been approved by their service managers, giving their previous approval to work in a room of level P2.
The cell culture room P2 is equipped with the basic material necessary to obtain and maintain cell cultures in a laboratory.



2. EQUIPMENT

The Cell Culture Service of the CABD is equipped by the following instruments:  
 


    1.    Two biological safety cabinets type II: Bio II advance 3 of Telstar, one of which is exclusively for work with viruses.
    
  • Two cell culture incubators (CO2):


    2.    CO2 Incubator HERAcell 150i : It is used as a quarantine incubator
   


    3.    CO2 Incubator Nuaire NU58416                                                                



 
 


    4.    CO2 installation: The equipment of the incubators is complemented by a CO2 installation that ensures the constant supply of CO2 to the incubators, making an automatic change of the bottles.
    



 


    5.    Tabletop centrifuge multitasking - Allegra X-12 (BECKMAN COULTER): maximum speed 10.200 rpm. Maximum capacity 3.000 ml.
    


 


    6.    Automatic hand cell counter (Millipore)
    


 


    7.    An tabletop autoclave and a bathroom.
    

    8. An inverted microscope of transmitted light with 10x, 20x and 40x lenses, Leica brand.
 
   
  9. A vacuum pump.
 

  10. A refrigerator / freezer
 


b.    Celldiscoverer 7 (Automated Microscopy Platform for Live Cell Imaging)

  
Inverted microscope, fully automated with C02 control, temperature and humidity. Thanks to its double set of lenses, it allows an amplification of the sample from 2.5x to 100x. It has lighting using LED technology in transmitted light and fluorescence. These features allow the observation and quantification of cell behavior over time by collecting and analyzing the images throughout the day automatically. It allows the contrast of the high definition phase, green fluorescence and red fluorescence.
 
Studies of phagocytosis, apoptosis, chemotaxis, cell migration and invasion, and cytotoxicity, monitoring of stem cells, efficiency of transfection and efficiency of cloning can be carried out.

 
Celldiscoverer 7 features

1 Microscope

• ZEISS Celldiscoverer 7
• Automatic sample container recognition
• Barcode reader
• Focus stabilization
• Magnification changer 0.5x / 1x / 2x
• Apochromatic FL beampath with adaptive field stop
• ZEISS Axiocam 506 mono
• Additional camera port
• On-axis access for dispensing
• UV-disinfection

2 Objectives

• Plan-APOCHROMAT 5x / 0.35
• Plan-APOCHROMAT 20x / 0.7 autocorr
• Plan-APOCHROMAT 20x / 0.95 autocorr
• Plan-APOCHROMAT 50x / 1.2 W autocorr
Autoimmersion

3 Illumination

• Transmitted light unit:
IR-LED (725 nm) brightfield, oblique illumination,
phase gradient contrast
• Fluorescence:
LEDs 385, 420, 470, 520, 567, 590 and 625 nm
High-efficiency multibandpass filter sets
Additional user-accessible emission filter Wheel

4 Accessories

• Temperature and atmospheric control
(heating / cooling; CO2, O2)
• Mounting frames for microwell plates,
incubations and perfusion
• Insert plates for dishes, multi-chamber slides
and standard slides

5 Software

• ZEN 2.3 celldiscoverer includes modules
for multi-dimensional image acqusition,
Tiles & Positions, Experiment Designer,
advanced image processing and analysis
tools



CONTACTO 


Contact with Corín Díaz Ramos
Phone: +34 954977432
E-mail: cdiaram(at)upo(dot)es