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Eduardo Santero CV

PUBLICATIONS

(In the last 10 years)
     
Mesa-Pereira B, Medina C, Camacho EM, Flores A, Santero E. 2014.
Improved cytotoxic effects of Salmonella-producing cytosine deaminase in tumour cells.
Microbial Biotechnology DOI:  10.1111/1751-7915.12153.

Terrón-González, L., Genilloud, O., Santero, E. 2014
Potential and limitations of metagenomic functional analyses. Chapter 1. Pp. 1-43. In: Metagenomics, Methods, Applications and Perspectives. Benedetti, C. (Ed.). Nova Publishers, New York. ISBN: 978-63321-698-3 (eBook).

Valentini M, Garcia-Mauriño SM, Pérez-Martinez I, Santero E, Canosa I, Lapouge K. 2014.
Hierarchical management of carbon sources is regulated similarly by the CbrA/B system in Pseudomonas aeruginosa and Pseudomonas putida.
Microbiology    DOI: 10.1099/mic.0.078873-0.

Platero, A, Santero, E, Govantes, F. 2014.
Genetic evidence of a high-affinity cyanuric acid transport system in Pseudomonas sp. ADP.
FEMS Microbiol. Letters, DOI: 10.1111/1574-6968.12392

Tomás-Gallardo L, Gómez-Álvarez H, Santero, E, Floriano B. 2014.
Combination of degradation pathways for naphthalene utilization in Rhodococcus sp. strain TFB.
Microbial Biotechnol., 7: 100-103 DOI: 10.1111/1751-7915.12096

Mesa-Pereira B, Medina C, Camacho EM, Flores A, Santero E. 2013.
Novel Tools to Analyze the Function of Salmonella Effectors Show That SvpB Ectopic Expression Induces Cell Cycle Arrest in Tumor Cells.
PLoS One, 8:e78458. doi: 10.1371/journal.pone.0078458.                   

Porrúa O, López-Sánchez A, Platero AI, Santero E, Shingler V, Govantes F. 2013.
An A-tract at the AtzR binding site assists DNA binding, inducer-dependent repositioning and transcriptional activation of the PatzDEF promoter.
Mol Microbiol. 90:72-87. doi: 10.1111/mmi.12346.       

Ledesma-García L, Reyes-Ramírez F, Santero E. 2013.
The ferredoxin ThnA3 negatively regulates tetralin biodegradation gene expression via ThnY, a ferredoxin reductase that functions as a regulator of the catabolic pathway.
PLoS One, 8:e73910. doi: 10.1371/journal.pone.0073910.                   

García-Mauriño SM, Pérez-Martínez I, Amador CI, Canosa I, Santero E. 2013.
Transcriptional activation of the CrcZ and CrcY regulatory RNAs by the CbrB response regulator in Pseudomonas putida.
Mol Microbiol., 89: 189-205.                                   

Terrón, L., Medina, C., Limón-Mortés, MC., Santero, E. 2013.
Heterologous viral expression systems in fosmid vectors increase the functional analysis potential of metagenomic libraries.
Scientific Reports, 3: 1107.                                   

Platero, A.I., García-Jaramillo, M., Santero, E., Govantes, F. 2012.
Transcriptional Organization and Regulatory Elements of a Pseudomonas sp. Strain ADP Operon Encoding a LysR-Type Regulator and a Putative Solute Transport System.
J. Bacteriol. 194(23): 6560-73.                               

Tomás-Gallardo, L., Santero, E., Floriano, B. 2012.
Involvement of a putative cyclic amp receptor protein (CRP)-like binding sequence and a CRP-like protein in glucose-mediated catabolite repression of thn genes in Rhodococcus sp. strain TFB.
Appl. Environ. Microbiol. 78(15):5460-2.                           

Medina, C., Santero, E., Gómez-Skarmeta, J.L., Royo, J.L. 2012.
Engineered Salmonella allows real-time heterologous gene expression monitoring within infected zebrafish embryos. J. Biotechnol. 157(3):413-6.                   

Santero, E., Hervás, A., Canosa, I., Govantes, F. 2012. Glutamate Dehydrogenases: Enzymology, Physiological Role and Biotechnological Relevance. Chapter 12. In: “Dehydrogenases”. RA Canuto (Ed). InTech. ISBN 978-953-307-019-3.                                   

Martín-Cabello, G., Moreno-Ruiz, E., Morales, V., Floriano, B., Santero, E. 2011.
Involvement of poly(3-hydroxybutyrate) synthesis in catabolite repression of tetralin biodegradation genes in Sphingomonas macrogolitabida strain TFA.
Environ. Microbiol. Reports, 3: 627-631.                           

Manuel Ferrer, María-Eugenia Guazzaroni, Michael Richter, Adela García-Salamanca, Pablo Yarza, Ana Suárez-Suárez, Jennifer Solano, María Alcaide, Pieter van Dillewijn, Maria Antonia Molina-Henares, Nieves López-Cortés, Yamal Al-Ramahi, Carmen Guerrero, Alejandro Acosta, Laura I. de Eugenio, Virginia Martínez, Silvia Marques, Fernando Rojo, Eduardo Santero, Olga Genilloud, Julian Pérez-Pérez, Ramón Rosselló-Móra, Juan Luis Ramos. 2011.
Taxonomic and Functional Metagenomic Profiling of the Microbial Community in the Anoxic Sediment of a Sub-saline Shallow Lake (Laguna de Carrizo, Central Spain)
Microbial Ecology, 62: 824-837                               

Medina, C., Camacho, E.M., Flores, A., Mesa-Pereira, B., Santero, E. 2011.
Improved Expression Systems for Regulated Expression in Salmonella Infecting Eukaryotic Cells
PLOSone, 6: e23055.                                       

Ledesma-García, L. Rivas-Marín, E., Floriano, B., Bernhardt, R., Ewen, K. M., Reyes-Ramírez, F., Santero, E. 2011
ThnY is a ferredoxin reductase-like iron-sulfur flavoprotein that has evolved to function as a regulator of tetralin biodegradation genes expression.
J. Biol. Chem., 286: 1709-1718.                               

Hervás AB, Canosa I, Santero E. 2010.
Regulation of glutamate dehydrogenase expression in Pseudomonas putida results from its direct repression by NtrC under nitrogen-limiting conditions.
Molecular Microbiology, 78: 305-319                           

Govantes F, García-González V, Porrúa O, Platero AI, Jiménez-Fernández A, Santero E. 2010.
Regulation of the atrazine-degradative genes in Pseudomonas sp. strain ADP.
FEMS Microbiol. Lett., 310: 1-8                               

Porrúa O, Platero AI, Santero E, Del Solar G, Govantes F. 2010.
Complex interplay between the LysR-type regulator AtzR and its binding site mediates atzDEF activation in response to two distinct signals.
Molecular Microbiology, 76: 331-347                           

Amador, C. I.; Canosa, I.; Govantes, F., Santero, E. 2010.
Lack of CbrB in Pseudomonas putida affects not only amino acids metabolism but also different stress responses and biofilm development
Environmental Microbiology, 12: 1748-1761                        

López-Sánchez, A., Floriano, B., Andújar, E., Hernáez, M.J., Santero, E. 2010.
Tetralin-induced and ThnR regulated aldehyde dehydrogenase and ?eta-oxidation genes in Sphingomonas macrogolitabida strain TFA.
Applied & Environmental Microbiology, 76: 110-118                     

Vicente García-González, Alicia Jiménez-Fernandez, Ana B. Hervas, Inés Canosa, Eduardo Santero & Fernando Govantes. 2009.
Distinct roles for NtrC and GlnK in nitrogen regulation of the Pseudomonas sp. Strain ADP cyanuric acid utilization operon.
FEMS Microbiology Letters, 300: 222-229                             

Hervás AB, Canosa I, Little R, Dixon R, Santero E. 2009.
NtrC-dependent regulatory network for nitrogen assimilation in Pseudomonas putida.
Journal of Bacteriology,191: 6123-6135                           

A. López-Sánchez, E. Rivas-Marín, O. Martínez-Pérez, B. Floriano & E. Santero. 2009.
Co-ordinated regulation of two divergent promoters through higher-order complex formation by the LysR-type regulator ThnR
Molecular Microbiology, 73: 1086- 1100                            

O. Porrúa, V. García-González, E. Santero & F. Govantes. 2009.
Activation and repression of a N-dependent promoter naturally lacking upstream activation sequences.
Molecular Microbiology, 73:  419- 433                           

F. Govantes, O. Porrúa, V. García-González & E. Santero. 2009.
Atrazine biodegradation in the lab and in the field: enzymatic activities and gene regulation
Microbial Biotechnology, 2: 178- 185                           

L. Tomás, E. Santero & B. Floriano. 2009.
Molecular and biochemical characterization of the tetralin degradation pathway in Rhodococcus sp. strain TFB
Microbial Biotechnology, 2: 262- 273                             

A. B. Hervás, I. Canosa & E. Santero. 2008.
Transcriptome analysis of Pseudomonas putida in response to nitrogen availability
Journal of Bacteriology, 190:416- 420                           

V. García-González, F. Govantes, A. Hervás, I. Canosa, O. Porrúa & E. Santero. 2007.
Regulation of the Atrazine degradative pathway in Pseudomonas. In “Bioremediation of Soils Contaminated with Aromatic Compounds”. NATO Science Series IV. Earth and Environmental Sciences, 76: 31-39. Springer. Hermann Heipieper, Editor. ISBN: 978-1-4020-5692-5        BC

O. Martínez-Pérez, A. López-Sánchez, F. Reyes-Ramírez, B. Floriano & E. Santero. 2007.
Integrated response to inducers by communication between a catabolic pathway and its regulatory system
Journal of Bacteriology, 189: 3768- 3775                           

O. Porrúa, M. García-Jaramillo, E. Santero & F. Govantes. 2007.
The LysR-type regulator AtzR binding site: DNA sequences involved in activation, repression and cyanuric acid-dependent repositioning
Molecular Microbiology, 62: 410- 427                           

J.L. Royo, P.D. Becker, E.M. Camacho, A. Cebolla, C. Link, E. Santero & C.A Guzmán. 2007.
In vivo gene regulation in Salmonella spp. by a salicylate-dependent control circuit
Nature Methods, 4:   937-  942                               

L. Yuste, A. Hervás, I. Canosa, R. Tobes, JI. Jiménez, J. Nogales, MM. Pérez-Pérez, E. Santero, E. Díaz, JL. Ramos, V. de Lorenzo & F. Rojo. 2006.
Growth phase-dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome-wide DNA microarray
Environmental Microbiology, 8: 165- 177                           

L. Tomás-Gallardo, I. Canosa, E. Santero, E. Camafeita, E. Calvo, JA. López & B. Floriano. 2006
Proteomic and transcriptional characterization of aromatic degradation pathways in Rhodoccocus sp strain TFB. Proteomics, 6: S119- S132                        A

V. García-González, F. Govantes, O. Porrúa, and E. Santero. 2005
Regulation of the Pseudomonas sp. Strain ADP Cyanuric Acid Degradation Operon
Journal of Bacteriology, 187: 155-167                           

JL. Royo, E. Moreno-Ruiz, A. Cebolla & E. Santero. 2005
Stable long-term indigo production by overexpression of dioxygenase genes using a chromosomal integrated cascade expression circuit
J. Biotechnology, 116: 113- 124                               

JL. Royo, H. Manyani, A. Cebolla & E. Santero. 2005.
A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation
Nucleic Acids Research, 33: e169                                



PATENTS



INVENTORS: Saadeding, A., Gómez del Pulgar, E., Santero, E. & Terrón, L.
TITLE: Polypeptides with cellulase activity
REF: P201430313 (Spanish patent)
PRIORITY DATE: March 7, 2014
HOLDER: Abengoa Research, S. L

INVENTORS: Terrón, L., Limón, Mª C & Santero E
TITLE: Heterologous expression systems for the functional analyses of metagenomic libraries
REF: PCT/ES2010/070761
PRIORITY DATE: November 22, 2010
HOLDER: Universidad Pablo de Olavide

INVENTORS: E. Santero, A. Cebolla & J.L. Royo
TITLE: Control of gene expression with the use of a transcription attenuator
REF.: PCT/ES2005000541
PRIORITY DATE: November 2004
HOLDER: Universidad Pablo de Olavide

INVENTORS: A. Cebolla, J. L. Royo & E. Santero
TITLE: Method of regulating heterologous protein expression controlled by salicylic derivatives in micro-organisms associated with higher organisms.
REF:    PCT/ES2004000528
PRIORITY DATE: December 2003
HOLDER: Universidad Pablo de Olavide


SUPERVISED PhD THESIS (completed in the last 10 years)




TITLE: Caracterización de la activación coordinada de los promotores divergentes PB-PC de los genes thn en Sphingopyxis macrogolitabida estirpe TFA
PHD STUDENT:  Elena Rivas Marín
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2014    RATE:  Sobresaliente cum laude


TITLE: Desarrollo de sistemas de expresión para análisis metagenómicos fucionales e identificación de enzimas de interés.
PHD STUDENT:  Laura Terrón González
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2014    RATE:  Sobresaliente cum laude

TITLE: Represión catabólica de la ruta de degradación de tetralina en Sphingopyxis macrogoltabida TFA
PHD STUDENT:  Helena Gómez Álvarez
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2014    RATE:  Sobresaliente cum laude

TITLE: Caracterización del sistema de dos componentes CbrAB en Pseudomonas putida KT2442
PHD STUDENT:  Sofía Muñoz García-Mauriño
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2014    RATE:  Sobresaliente cum laude

TITLE: Caracterización genética y funcional del operón atzRSTUVW de Pseudomonas sp. ADP
PhD STUDENT:  Ana Isabel Platero
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2013    QUALIFICATION:  Sobresaliente cum laude

TITLE: Caracterización funcional de ThnY y de la ferredoxina ThnA3 en la regulación de los genes
de degradación de tetralina de Sphingomonas macrogolitabida estirpe TFA.
PhD STUDENT:  Laura Ledesma García
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2012    RATE:  Sobresaliente cum laude

TITLE: Caracterización del papel regulador de CbrB en Pseudomonas putida
PhD STUDENT:  Cristina Amador Hierro
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2012    RATE:  Sobresaliente cum laude

TITLE: Utilización de Salmonellas atenuadas como sistemas de producción de agentes antitumorales
PhD STUDENT:  Beatriz Mesa Pereira
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:    2012    RATE:  Sobresaliente cum laude

TITLE: Estudio de la Regulación por Nitrógeno en Pseudomonas putida.
PhD STUDENT:  Ana Belén Hervás Veguillas
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:  2011        RATE:  Sobresaliente cum laude

TITLE: Degradación de compuestos aromáticos en Rhodococcus sp. estirpe TFB.
PhD STUDENT: Laura Tomás Gallardo
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:  2009        RATE:  Sobresaliente cum laude

TITLE: Characterization of the regulatory mechanisms od atzR and atzDEF expresión by the LysR-type regulador AtzR.
PhD STUDENT:  Odil Porrúa Fuertes
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:  2009        RATE:  Sobresaliente cum laude

TITLE: Expresión de lso genes thn: organización transcripcional y activación mediada por el regulador tipo LysR ThnR.
PhD STUDENT:  Aroa López Sánchez
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:  2009        RATE:  Sobresaliente cum laude

TITLE: Regulación de los genes de degradación de tetralina
PhD STUDENT:  Olga Martínez Pérez
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:  2008        RATE:  Sobresaliente cum laude

TITLE: Regulación por nitrógeno del metabolismo de atrazina en Pseudomonas sp. ADP
PhD STUDENT:  Vicente Marco García González
UNIVERSITY: Pablo de Olavide    FACULTY: Ciencias Experimentales
YEAR:  2006        RATE:  Sobresaliente cum laude